Dynamic DNA methylation

A SNF2-like protein faciliates dynamic control of DNA methylation

Supplementary Methods Primers used for genotyping: The α’ promoter silencing system and the drd1 mutant alleles have been described previously (Kanno et al., 2004). The work reported here was carried out using plants carrying the drd1-6 allele. The drd1-6 mutation produces a G to A substitution (TGGTCA to TGATCA), producing a new NdeII restriction enzyme site (/GATC) that can be used for genoty...

Dynamic Changes in DNA Methylation in Ischemic Tolerance

Epigenetic mediators of gene expression are hypothesized to regulate transcriptomic responses to preconditioning ischemia and ischemic tolerance. Here, we utilized a methyl-DNA enrichment protocol and sequencing (ChIP-seq) to identify patterns of DNA methylation in an established model of ischemic tolerance in neuronal cultures (oxygen and glucose deprivation: OGD). We observed an overall decre...

Convergent Transcription Induces Dynamic DNA Methylation at disiRNA Loci

Cytosine methylation of DNA is an important epigenetic gene silencing mechanism in plants, fungi, and animals. In the filamentous fungus Neurospora crassa, nearly all known DNA methylations occur in transposon relics and repetitive sequences, and DNA methylation does not depend on the canonical RNAi pathway. disiRNAs are Dicer-independent small non-coding RNAs that arise from gene-rich part of ...

Dynamic DNA methylation across diverse human cell lines and tissues.

As studies of DNA methylation increase in scope, it has become evident that methylation has a complex relationship with gene expression, plays an important role in defining cell types, and is disrupted in many diseases. We describe large-scale single-base resolution DNA methylation profiling on a diverse collection of 82 human cell lines and tissues using reduced representation bisulfite sequen...

Evidence that DNA methylation engenders dynamic gene regulation.